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KEPI Antibody / Kinase-enhanced PP1 inhibitor / PPP1R14C

Protein phosphatase 1 regulatory subunit 14C, also called Kinase-enhanced PP1 inhibitor (KEPI), is an enzyme that in humans is encoded by the PPP1R14C gene. The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8TAE6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids H67-V165) was used as the immunogen for the KEPI antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the KEPI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This KEPI antibody is available for research use only.

Storage Conditions

After reconstitution, the KEPI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the KEPI antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human 293T cells with KEPI antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= KEPI antibody.

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