NEIL3 Antibody / Endonuclease 8-like 3

Nei endonuclease VIII-like 3 (E. coli) is a protein in humans that is encoded by the NEIL3 gene. NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8TAT5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids K244-K585) was used as the immunogen for the NEIL3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NEIL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NEIL3 antibody is available for research use only.

Storage Conditions

After reconstitution, the NEIL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NEIL3 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human thyroid papillary carcinoma tissue with NEIL3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.