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ROBO4 Antibody / Roundabout homolog 4

Roundabout homolog 4 is a protein that in humans is encoded by the ROBO4 gene. Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8WZ75

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids D108-A933) was used as the immunogen for the ROBO4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ROBO4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ROBO4 antibody is available for research use only.

Storage Conditions

After reconstitution, the ROBO4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ROBO4 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human HeLa, 2) human HepG2, 3) rat PC-12 and 4) mouse LLC cell lysate with ROBO4 antibody. Predicted molecular weight: 75-107 kDa (multiple isoforms), but may be observed at higher molecular weights due to glycosylation.

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