Selenocysteine lyase Antibody / SCLY

SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al. (2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96I15

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids E157-A445) was used as the immunogen for the Selenocysteine lyase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Selenocysteine lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Selenocysteine lyase antibody is available for research use only.

Storage Conditions

After reconstitution, the Selenocysteine lyase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Selenocysteine lyase antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human rectum adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.