Ephrin Receptor B3 Antibody / EPHB3

Ephrin Receptor EphB3, is also known as human embryo kinase 2 (HEK2) or Eph-like tyrosine kinase 2 (ETK2) . EPHB3, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. EPHB3/HEK2 interacts with 2 ligands of EPH-related kinases (LERKs), namely, LERK2 (EFNB1) and LERK5 (EFNB2) . Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of EPHB3/HEK2 and LERK2 results in reduced kinase activity of EPHB3/HEK2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P54753

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids H275-Q554) was used as the immunogen for the Ephrin Receptor B3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Ephrin Receptor B3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ephrin Receptor B3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ephrin Receptor B3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Ephrin Receptor B3 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human glioma tissue with Ephrin Receptor B3 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.