Eukaryotic translation initiation factor 5 Antibody / EIF5

Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P55010

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids D11-D423) was used as the immunogen for the Eukaryotic translation initiation factor 5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Eukaryotic translation initiation factor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Eukaryotic translation initiation factor 5 antibody is available for research use only.

Storage Conditions

After reconstitution, the Eukaryotic translation initiation factor 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Eukaryotic translation initiation factor 5 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.