PKCE Antibody / PKC epsilon / PRKCE

Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q02156

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids M1-K690) was used as the immunogen for the PKCE antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PKCE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PKCE antibody is available for research use only.

Storage Conditions

After reconstitution, the PKCE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PKCE antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human A549 cells with PKCE antibody (green) and Alpha Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.