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NAAA Antibody / N-acylethanolamine-hydrolyzing acid amidase

N-acylethanolamine-hydrolyzing acid amidase, also called ASAHL and PLT, is an enzyme that in humans is encoded by the NAAA gene. Enables N- (long-chain-acyl) ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q02083

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R35-L325) was used as the immunogen for the NAAA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NAAA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NAAA antibody is available for research use only.

Storage Conditions

After reconstitution, the NAAA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NAAA antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human JK cells with NAAA antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= NAAA antibody.

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