POLR3GL Antibody / RPC32 beta

Polymerase (RNA) III (DNA directed) polypeptide G (32kD) -like, also known as RNA polymerase III 32 kDa beta subunit, is a protein which in humans is encoded by the POLR3GL gene. Predicted to enable chromatin binding activity. Involved in transcription by RNA polymerase III. Located in nucleus. Part of RNA polymerase III complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9BT43

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids R8-K156) was used as the immunogen for the POLR3GL antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the POLR3GL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This POLR3GL antibody is available for research use only.

Storage Conditions

After reconstitution, the POLR3GL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the POLR3GL antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with POLR3GL antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.