OTT1 Antibody / One-twenty two protein 1 / RBM15

Putative RNA-binding protein 15, also called One-twenty two protein 1 (OTT1), is a protein that in humans is encoded by the RBM15 gene. Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96T37

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids M1-K967) was used as the immunogen for the OTT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the OTT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This OTT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the OTT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the OTT1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human prostate cancer tissue with OTT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.