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Glucosidase 2 subunit beta Antibody / PRKCSH

Glucosidase 2 subunit beta is an enzyme that in humans is encoded by the PRKCSH gene. This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P14314

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids E16-L506) was used as the immunogen for the Glucosidase 2 subunit beta antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Glucosidase 2 subunit beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Glucosidase 2 subunit beta antibody is available for research use only.

Storage Conditions

After reconstitution, the Glucosidase 2 subunit beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Glucosidase 2 subunit beta antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A549 cells with Glucosidase 2 subunit beta antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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