PAN2 Antibody / USP52

PAB-dependent poly (A) -specific ribonuclease subunit 2, also called Inactive ubiquitin carboxyl-terminal hydrolase 52 (USP52), is an enzyme that in humans is encoded by the PAN2 gene. This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly (A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q504Q3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids M1-L356) was used as the immunogen for the PAN2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PAN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PAN2 antibody is available for research use only.

Storage Conditions

After reconstitution, the PAN2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PAN2 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human PC-3 cells with PAN2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PAN2 antibody.