Anti-DR4 (NT) Antibody

Rabbit Polyclonal Antibody specific to DR4 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD antigen CD261

Gene Name

TNFRSF10A

Gene ID

8797

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=TNFRSF10A">TNFRSF10A</a>

UniProt

O00220

Accession Number

NP_003835.3

Cellular Locus

Cell membrane, Membrane raft, Cytoplasm, cytosol

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 1-20 of human DR4 mature protein (accession no. ACC51226).

Target Antigen

Tumor necrosis factor receptor superfamily member 10A

Target

DR4 (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IF, ICC

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789). Promotes the activation of NF-kappa-B (PubMed:9430227). {PubMed:19090789, PubMed:9430227}.

Additionnal Information

Immunoblotting : use at 2ug/mL. Positive control: Whole cell lysate from HeLa, K562, or Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentration for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human DR4 (57kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. Another cell death receptor was recently identified and designated DR4 (for death receptor 4). The ligand for DR4 has been designated TRAIL and is a new member of the TNF family. Therefore, DR4 is also called TRAIL receptor-1. DR4 is expressed in most human tissues including spleen, peripheral blood, small intestine, and thymus. Like TNFR1, Fas and DR3, DR4 mediates TRAIL-induced apoptosis and NF- B activation in many tissues and cells.