Bulk Human IgG4 (IB4) Isotype Control SPLEPG
Product Specifications
Background
Recombinant IgG4 human antibody
Specifications
This Human IgG4 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG1 antibody is suitable for in vivo applications and each lot is IMPACT I certified.
Host
CHO
Species Reactivity
Human
Target
IgG4
Isotype
IgG4
Clone
IB4
Applications
Flow Cytometry, Functional Assays
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Concentration
≥ 5.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC, >95% by SDS Page
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
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