Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin
Selected Variant
ICH1203-5mg
Size: 5 mg
Product Specifications
CAS Number
9007-83-4
Specifications
Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v.
Host
Mouse
Species Reactivity
Mouse
Target
MHC Class I
Isotype
IgG2a κ
Clone
AF6-88.5
Applications
Functional Assays, Flow Cytometry, IHC Frozen, Immunoprecipitation
Purification Method
This monoclonal antibody was purified using Protein A
Concentration
≥ 2.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245
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