Anti-Mouse Ly49C (4LO3311) In Vivo Antibody - Low Endotoxin
Selected Variant
ICH1202-50mg
Size: 50 mg
Product Specifications
Background
4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.
CAS Number
9007-83-4
Specifications
4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1). 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.
Product Name Alternative
Ly-49 inhibitory receptors C
Host
Mouse
Species Reactivity
Mouse
Target
Ly49C
Isotype
IgG3 Kappa
Clone
4LO3311
Applications
Depletion, Flow Cytometry, Immunoprecipitation
Purification Method
This monoclonal antibody was purified using Protein A
Concentration
≥ 2.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution
Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Mouse-IgG3-Isotype-Control-for-In-Vivo-Low-Endotoxin-MG3-35-ICH2249
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