Anti-Mouse CD49d (PS/2) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.

CAS Number

9007-83-4

Specifications

PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.

Product Name Alternative

VLA-4α, ITGA4, Integrin α4

Host

Rat

Species Reactivity

Mouse,Human

Target

CD49d

Isotype

IgG2b Kappa

Clone

PS/2

Applications

Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://ichor.bio/product/rat-igg2b-in-vivo-isotype-control-low-endotoxin-1-2-ich2243/

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