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Mouse Anti-Mouse PD-L1 (10F.9G2) Recombinant Antibody

Product Specifications

Background

CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.

CAS Number

9000-83-3

Specifications

Mouse anti-mouse PD-L1 antibody (10F.9G2) is a recombinant antibody where the original rat IgG2b backbone of 10F.9G2 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse CD274

Product Name Alternative

Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1

UniProt

Q9EP73

Host

Mouse

Species Reactivity

Mouse

Target

PD-L1

Isotype

IgG1

Clone

10F.9G2

Applications

Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Mouse Anti-mouse PD-L1 antibody (10F.9G2) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD274 antigien is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247

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