Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin
Product Specifications
CAS Number
9007-83-4
Specifications
Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site.
Product Name Alternative
CD159a, CD159c, CD159e, NKG2C, NKG2E
Host
Rat
Species Reactivity
Mouse
Target
NKG2A/C/E
Isotype
IgG2a
Clone
20D5
Applications
Flow Cytometry
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Antigen Distribution
NK cells and NK T cells from C57BL/6 mouse
Aggregation
Aggregation level ≤ 5%
Applications Notes
For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.
Isotype Control
www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
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