Anti-Mouse TCR V Gamma 2 (UC3-10A6) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an alpha and beta chain in 95% of T cells, while 5% of T cells have TCRs consisting of gamma and delta chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

CAS Number

9007-83-4

Specifications

This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes.

Product Name Alternative

V gamma 2

Host

Armenian Hamster

Species Reactivity

Mouse

Target

TCR V gamma 2

Isotype

IgG

Clone

UC3-10A6

Applications

Flow Cytometry, Immunoprecipitation, In vivo Depletion

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-TCR V gamma 2 In Vivo Antibody - Low Endotoxin (UC3-10A6) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

V gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of gamma delta TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat Goat Anti-Armenian Hamster IgG (H&L)-R-phycoerythrin Each investigator should determine their own optimal working dilution for specific applications.