Anti-Mouse IFN Alpha (TIF-3C5) In Vivo Antibody - Ultra-Low Endotoxin

Anti-IFN alpha In Vivo Antibody - Ultra-Low Endotoxin (TIF-3C5) recognizes an epitope on Mouse IFNalpha and binds specifically to murine IFNa species (IFN-alpha-A, -1, -4, -5, -11. and -13) and does not bind murine murine IFNg or IFNb

Anti-IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) is stable when stored at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Functional Assay: In vitro activity of TIF-3C5 - inhibits IFNalpha -induced (IFNalpha-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNA (IFNA-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNA-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNalpha-A, -1, -4, -5, -11 and 13) and natural IFNalpha can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice. Each investigator should determine their own optimal working dilution for specific applications.