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Anti-CD45R (RA3-6B2) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Background

CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

Specifications

Anti-CD45R In Vivo Antibody - Ultra Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.

Product Name Alternative

Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220

UniProt

P08575

Host

Rat

Species Reactivity

Mouse,Human

Target

CD45R

Isotype

IgG2a

Clone

RA3-6B2

Applications

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD45R In Vivo Antibody - Ultra Low Endotoxin (RA3-6B2) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Human-CD45R-In-Vivo-Antibody-Low-Endotoxin-RA3-6B2-ICH1130/

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .

Aggregation

Aggregation level ≤ 1%

Applications Notes

Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

CAS Number

9007-83-4

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