Anti-Mouse GM-CSF (MP1-22E9) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.
CAS Number
9007-83-4
Specifications
Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF
Product Name Alternative
Csf2, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Csfgm
UniProt
P01587
Host
Rat
Species Reactivity
Mouse
Target
GM-CSF
Isotype
IgG2a
Clone
MP1-22E9
Applications
Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Formulation
0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml.Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
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