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Anti-Mouse CD44 (IM7) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Background

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Clone IM7 antibody has been shown to neutralize CD44 in vivo

Specifications

Anti-CD44 In Vivo Antibody - Ultra Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186

Product Name Alternative

Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I

UniProt

P15379

Host

Rat

Species Reactivity

Mouse

Target

CD44

Isotype

IgG2b

Clone

IM7

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen), ELISA, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD44 In Vivo Antibody - Ultra Low Endotoxin (IM7) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-CD44-In-Vivo-Antibody-Low-Endotoxin-IM7-ICH1115/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reacts with: Ms+, Hu+, Bov+, Mky+, Bab+, Dog+, Cat, Porcine

Aggregation

Aggregation level ≤ 1%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

CAS Number

9007-83-4

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