Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin
Product Specifications
CAS Number
9007-83-4
Specifications
Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C
Product Name Alternative
Lymphocyte antigen 6 complex, locus C
UniProt
P0CW02
Host
Rat
Species Reactivity
Mouse
Target
Ly-6C
Isotype
IgG2c
Clone
HK1.4
Applications
Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen)
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
0.5 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Formulation
No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Antigen Distribution
The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Aggregation
Aggregation level ≤ 5%
Applications Notes
Immunoprecipitation: 1–2 μg per 100–500 μg of total protein (1 ml of cell lysate).
Each investigator should determine their own optimal working dilution for specific applications.
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