Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Low Endotoxin

Product Specifications

CAS Number

9007-83-4

Specifications

Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

Product Name Alternative

IFN-b, IFN-beta, Type-1 interferon, Interferon beta

UniProt

P01575

Host

Mouse

Species Reactivity

Mouse

Target

IFN beta

Isotype

IgG2a

Clone

HDB-4A7

Applications

Western Blot, Blocking, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days. Each investigator should determine their own optimal working dilution for specific applications.