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Anti-Mouse IFN Gamma (H22) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Background

Interferon-gamma (IFN-gamma) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-gamma has antiviral, immunoregulatory, and anti-tumour properties

Specifications

Anti-IFN gamma In Vivo Antibody - Ultra Low Endotoxin (H22) recognizes an epitope on Mouse IFN-gamma

Product Name Alternative

Interferon gamma, Ifng

UniProt

P01580

Host

Armenian Hamster

Species Reactivity

Mouse

Target

IFN gamma

Isotype

IgG

Clone

H22

Applications

Immunoprecipitation, Western Blot, Neutralization, ELISA, Immunofluorescence

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-IFN gamma In Vivo Antibody - Ultra Low Endotoxin (H22) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-IFN-gamma-In-Vivo-Antibody-Low-Endotoxin-H22-ICH1109/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide.

Aggregation

Aggregation level ≤ 1%

Applications Notes

Western Blotting: To detect Mouse IFN-gamma clone H22 can be used at a concentration of 0.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

CAS Number

9007-83-4

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