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Anti-Mouse Ly-6G (1A8) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Background

Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

CAS Number

9007-83-4

Specifications

Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin (1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C

Product Name Alternative

Lymphocyte antigen 6 complex, locus G

UniProt

P35461

Host

Rat

Species Reactivity

Mouse

Target

Ly-6G

Isotype

IgG2a

Clone

1A8

Applications

IHC (FFPE), IHC (Frozen), Flow Cytometry, Western Blot, In vivo Depletion, Immunfluorescence

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin (1A8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Aggregation

Aggregation level ≤ 1%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

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