Anti-Mouse CTLA-4 (UC10-4F10-11) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD152, also known as CTLA-4 or Ly-56 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

CAS Number

9007-83-4

Specifications

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) recognizes an epitope on Mouse CD152

Product Name Alternative

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

UniProt

P09793

Host

Armenian Hamster

Species Reactivity

Mouse

Target

CTLA-4

Isotype

IgG1 Kappa

Clone

UC10-4F10-11

Applications

Flow Cytometry, Western Blot, Functional Assays, Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) is stable when stored at 2-8°C. Do not freeze.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.4, without BSA or sodium azide added.

Antigen Distribution

The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: Clone UC10-4F10-11 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding. Each investigator should determine their own optimal working dilution for specific applications.