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Anti-Mouse LAG-3 (C9B7W) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

LAG-3, also known as CD223, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. Clone C9B7W antibody has been reported to block the function of murine LAG-3 in vivo and in vitro.

Specifications

Anti-LAG-3 In Vivo Antibody - Low Endotoxin (C9B7W) recognizes an epitope on Mouse LAG-3

Product Name Alternative

Lymphocyte activation gene 3 protein, Aida, CD223

UniProt

Q61790

Host

Rat

Species Reactivity

Mouse

Target

LAG3

Isotype

IgG1 Kappa

Clone

C9B7W

Applications

Western Blot, Blocking, Flow Cytometry, Immunoprecipitation, ELISA

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-LAG-3 In Vivo Antibody - Low Endotoxin (C9B7W) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-LAG-3-In-Vivo-Antibody-Low-Endotoxin-C9B7W-ICH1078/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

T-cells and NK cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Clone C9B7W antibody blocks the function of murine LAG-3 in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246

CAS Number

9007-83-4

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