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Anti-Mouse CD172a (P84) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Signal regulatory protein alpha (SIRPalpha), also known as CD172a, is a regulatory membrane type I glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.

Specifications

Anti-CD172a In Vivo Antibody - Low Endotoxin (P84) recognizes an epitope on Mouse CD172a

Product Name Alternative

Sirpa, Sirp-alpha-1, SHP substrate 1, SHPS-1, Shps1, Bit, p84, MyD-1 antigen, Myd1, Ptpns1

UniProt

P97797

Host

Rat

Species Reactivity

Mouse

Target

CD172a

Isotype

IgG1 Kappa

Clone

P84

Applications

Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD172a In Vivo Antibody - Low Endotoxin (P84) is stable when stored at 2-8°C. Do not freeze.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/anti-cd172a-in-vivo-antibody-low-endotoxin-p84-ich1076/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Monocytes, macrophages, myeloid cells, neuronal tissue

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Reported applications in literature include: blocking SIRPalpha interaction with CD47 & in vivo blocking of dendritic cell migration. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246

CAS Number

9007-83-4

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