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Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, and LAM-1. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.

Specifications

Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) recognizes an epitope on Mouse CD62L

Product Name Alternative

L-selectin, Sell, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1 Lymph node homing receptor1, Lymphocyte antigen 221, Ly-221, Lymphocyte surface MEL-14 antigen, Lnhr, Ly-22, Ly22

UniProt

P18337

Host

Rat

Species Reactivity

Mouse

Target

CD62L

Isotype

IgG2a

Clone

MEL-14

Applications

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen), CyTOF

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/anti-cd62l-mel-14-in-vivo-antibody-low-endotoxin/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

CAS Number

9007-83-4

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