Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin

CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma.

Anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.

Anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

20 mM Histidine pH 6.0 containing 150 mM NaCl. NOTE: A buffer exchange into PBS for conjugation and MES for functional assays may be required depending on your application.

Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells. Clone 37.51 antibody has been shown to stimulate the proliferation and cytokine production by activated T and NK cells and provide a costimulatory signal for CTL induction.

Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each investigator should determine their own optimal working dilution for specific applications.