Anti-Mouse CD25 (PC61) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation. IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.

CAS Number

9007-83-4

Specifications

Anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA

Product Name Alternative

Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, Il2ra, p55, CD25

UniProt

P01590

Host

Rat

Species Reactivity

Mouse

Target

IL2RA

Isotype

IgG1 Lambda

Clone

PC61

Applications

Immunoprecipitation, Western Blot, Functional Assays, Blocking, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B cells. The CD25 antigen is negative on CD 56 positive natural killer cells, CD 14 positive monocytes, granulocytes, red blood cells and platlets. The CD25 antigen is expressed on malignant cells of adult T-cell leukemias (ATL's) carring HTLV-1, most cases of cutaneous T cell lymphoma and lymphoepithelioid lymphomas among non-Hodgkin's T cell lymphomas.