Anti-Mouse CD11a (FD441.8) In Vivo Antibody - Low Endotoxin
Product Specifications
CAS Number
9007-83-4
Specifications
Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) recognizes an epitope on Mouse CD11a
Product Name Alternative
LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL, Ly-15
UniProt
P24063
Host
Rat
Species Reactivity
Mouse
Target
CD11a
Isotype
IgG2b
Clone
FD441.8
Applications
Western Blot, Functional Assays, Flow Cytometry
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Functional Assay: The antibody inhibits ConA-facilitated lysis, but does not affect PHA-facilitated lysis.
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243
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