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Anti-Mouse CD8a (2.43) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Specifications

Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (2.43) recognizes an epitope on Mouse CD8a

Product Name Alternative

CD8 alpha chain, Lyt-2, Lyt2

UniProt

P01731

Host

Rat

Species Reactivity

Mouse

Target

CD8a

Isotype

IgG2b

Clone

2.43

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, Immunofluorescence, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (2.43) is stable when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-CD8a-In-Vivo-Antibody-Low-Endotoxin-2.43-ICH1045/

Formulation

This purified antibody is formulated with no carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

CD8a (Ly 2.2) is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 1%

Applications Notes

Functional Assay: In vivo Depletion: It has been reported that Balb/C mice may be injected intraperitoneally with 1.0 mg of Rat Anti-Mouse CD8a (Clone 2.43) antibody and allowed to rest for 24 hours for CD8+ T cell depletion.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

CAS Number

9007-83-4

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