Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.
CAS Number
9007-83-4
Specifications
Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Product Name Alternative
CD8 alpha chain, Lyt-2, Lyt2
UniProt
P01731
Host
Rat
Species Reactivity
Mouse
Target
CD8a
Isotype
IgG2a Kappa
Clone
53-6.7
Applications
Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins.
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
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