Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
CAS Number
9007-83-4
Specifications
Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Product Name Alternative
T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
UniProt
P06332
Host
Rat
Species Reactivity
Mouse
Target
CD4
Isotype
IgG2b Kappa
Clone
GK1.5
Applications
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
≥ 2.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243
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