Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.

CAS Number

9007-83-4

Specifications

Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4

Product Name Alternative

T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

UniProt

P06332

Host

Rat

Species Reactivity

Mouse

Target

CD4

Isotype

IgG2b Kappa

Clone

GK1.5

Applications

Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications.