Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.

CAS Number

9007-83-4

Specifications

Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε

Product Name Alternative

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

UniProt

P22646

Host

Syrian Hamster

Species Reactivity

Mouse

Target

CD3e

Clone

500A2

Applications

Functional Assays, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.