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Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4). CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.

Specifications

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

Product Name Alternative

Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1

UniProt

P42082

Host

Rat

Species Reactivity

Mouse

Target

CD86

Isotype

IgG2a Kappa

Clone

GL1

Applications

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/anti-cd86-gl1-in-vivo-antibody-low-endotoxin/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

CAS Number

9007-83-4

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