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Anti-Mouse TCR Gamma/Delta (UC7-13D5) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an alpha and beta chain in 95% of T cells, while 5% of T cells have TCRs consisting of gamma and delta chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Specifications

The anti-mouse γ/δ T-cell receptor antibody reacts with all mouse TCR γ/δ heterodimers.

Product Name Alternative

TCR gamma chain, TCR delta chain

Host

Armenian Hamster

Species Reactivity

Mouse

Target

TCR gamma/delta

Isotype

IgG

Clone

UC7-13D5

Applications

Flow Cytometry, Immunoprecipitation, In vivo Depletion

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-TCR gamma/delta In Vivo Antibody - Low Endotoxin (UC7-13D5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/delta-In-Vivo-Antibody-Low-Endotoxin-UC7-13D5-ICH1035/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

gamma/delta TCR expression is observed on a subset of cells in the thymus, intestinal epithelium, skin, liver, peripheral lymphoid tissues, and peritoneum. The gamma/delta TCR is involved in the antigen recognition of some bacterial or tumor-associated antigens presented by MHC class I.

Aggregation

Aggregation level ≤ 5%

Applications Notes

In vivo Depletion: This Clone UC7-13D5 has been used for the depletion of TCR gamma/delta T cells in vivo Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

CAS Number

9007-83-4

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