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Bulk Anti-Human HLA-DP (B7/21) Antibody

Product Specifications

CAS Number

9007-83-4

Specifications

Anti-HLA-DP In Vivo Antibody - Low Endotoxin (B7/21) recognizes a (Mr 26-34 kDa) class II major histocompatibility complex (MHC) antigen. The antibody recognizes a monomorphic determinant present on cells expressing DP1, DP2, DP3, DP4 and DP5.

Product Name Alternative

HLA-DP Monomorphic

Host

Mouse

Species Reactivity

Human

Target

HLA-DP

Isotype

IgG3

Clone

B7/21

Applications

Western Blot, Immunocytochemistry, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-HLA-DP In Vivo Antibody - Low Endotoxin (B7/21) is stable for at least six (6) weeks when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

Antigen Distribution

The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Mouse-IgG3-Isotype-Control-for-In-Vivo-Low-Endotoxin-MG3-35-ICH2249

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