Bulk Anti-Human MHC Class I (W6/32) Antibody

Product Specifications

Background

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surveillance.

CAS Number

9007-83-4

Specifications

Anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C

Product Name Alternative

Major Histocompatibility Class I, MHC class I

Host

Mouse

Species Reactivity

Human

Target

MHC Class I

Isotype

IgG2a

Clone

W6/32

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

No carrier proteins or preservatives in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl.

Antigen Distribution

This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

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