Bulk Anti-Human CD56 (ERIC-1) Antibody
Product Specifications
Background
Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested
Specifications
Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM)
Product Name Alternative
Neural cell adhesion molecule 1, NCAM1
UniProt
P13591
Host
Mouse
Species Reactivity
Human
Target
CD56
Isotype
IgG1
Clone
ERIC-1
Applications
ELISA Capture, IHC (Frozen), IHC (Paraffin), Western Blot, Immunoprecipitation, Dot Blot
Purification Method
This monoclonal antibody was purified using Protein A
Concentration
1.0 - 5.0 mg/mL
Endotoxin
<0.5 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Product Datasheet
https://ichor.bio/productpdf/print/index/url_key/bulk-anti-human-cd56-eric-1-antibody/
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Aggregation
Aggregation level ≤ 5%
Applications Notes
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 80 ng/mL.
Dot Blots: Immunoblotting: Recognizes 120, 140, and 180 kDa NCAM isoforms.
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247
CAS Number
9007-83-4
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