Bulk Anti-Human CD14 (UCHM-1) Antibody

Product Specifications

Background

Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes, B-cells, dendritic cells. CD14 functions to enhance activation of cells by lipopolysaccharide (LPS).

CAS Number

9007-83-4

Specifications

Anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes.

Product Name Alternative

Myeloid cell-specific leucine-rich glycoprotein

UniProt

P08571

Host

Mouse

Species Reactivity

Human

Target

CD14

Isotype

IgG2a

Clone

UCHM-1

Applications

Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and analyzed by silver stain.

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

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