Anti-Beta-Actin Antibody

Rabbit Polyclonal Antibody specific to Beta-Actin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Beta-actin [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]

Gene Name

ACTB

NCBI Gene ID

UniProt

P60709

Cellular Locus

Cytoplasm, cytoskeleton, Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to mid-protein amino acids of human beta-actin.

Target Antigen

Actin, cytoplasmic 1

Target

Beta-Actin

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). {PubMed:29581253, PubMed:29925947}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunofluorescence: use at 10ug/mL. These are recommended concentrations; Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human beta-actin.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Actins are essential components of the cytoskeleton with critical roles in a wide range of cellular processes including cell migration, cell division, and the regulation of gene expression. Beta-actin, product of the Actb gene, is one of six different actin isoforms and is one of the two nonmuscle cytoplasmic actins (gamma-actin is the other); both are ubiquitously expressed and highly conserved from birds to mammals. Mutations in the Actb gene have been associated with diffuse large B-cell lymphoma. Beta-actin (molecular weight ~42kDa) is often used as a loading control in Western blots.