What is the catalog number for Anti-Beta-Actin Antibody?

The catalog number for Anti-Beta-Actin Antibody is 56574-1.

What sizes are available for Anti-Beta-Actin Antibody?

Anti-Beta-Actin Antibody is available in the following size(s): 100 µg.

Is Anti-Beta-Actin Antibody in stock?

Anti-Beta-Actin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Anti-Beta-Actin Antibody

Rabbit Polyclonal Antibody specific to Beta-Actin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Beta-actin [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]

Gene Name

ACTB

NCBI Gene ID

UniProt

P60709

Cellular Locus

Cytoplasm, cytoskeleton, Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to mid-protein amino acids of human beta-actin.

Target Antigen

Actin, cytoplasmic 1

Target

Beta-Actin

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). {PubMed:29581253, PubMed:29925947}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunofluorescence: use at 10ug/mL. These are recommended concentrations; Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human beta-actin.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Actins are essential components of the cytoskeleton with critical roles in a wide range of cellular processes including cell migration, cell division, and the regulation of gene expression. Beta-actin, product of the Actb gene, is one of six different actin isoforms and is one of the two nonmuscle cytoplasmic actins (gamma-actin is the other); both are ubiquitously expressed and highly conserved from birds to mammals. Mutations in the Actb gene have been associated with diffuse large B-cell lymphoma. Beta-actin (molecular weight ~42kDa) is often used as a loading control in Western blots.