ONECUT1 Antibody / One cut homeobox 1 / HNF6

One cut homeobox 1 (ONECUT1), also called Hepatocyte nuclear factor 6 (HNF6), is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q9UBC0

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids M1-E270) was used as the immunogen for the ONECUT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ONECUT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ONECUT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the ONECUT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ONECUT1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with ONECUT1 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.