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4E-T Antibody / EIF4ENIF1

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q9NRA8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids D13-Q985) was used as the immunogen for the 4E-T antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the 4E-T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This 4E-T antibody is available for research use only.

Storage Conditions

After reconstitution, the 4E-T antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the 4E-T antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= 4E-T antibody.

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