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RUFY4 Antibody / RUN and FYVE domain-containing protein 4

Enables phosphatidylinositol-3-phosphate binding activity. Involved in autophagosome assembly; cellular response to interleukin-4; and positive regulation of macroautophagy. Located in autophagosome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q6ZNE9

Host

Rabbit

Reactivity

Human, Monkey

Immunogen

E. coli-derived recombinant human protein (amino acids M1-K553) was used as the immunogen for the RUFY4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the RUFY4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RUFY4 antibody is available for research use only.

Storage Conditions

After reconstitution, the RUFY4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RUFY4 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with RUFY4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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